Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization.
نویسندگان
چکیده
The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacterial probes. Nearly 45% of the total cells could be further identified as belonging to known phyla. Members of the Cytophaga-Flavobacterium cluster were most abundant in all layers, followed by the sulfate-reducing bacteria.
منابع مشابه
The effects of crude oil on marine microbial communities in sediments from the Persian Gulf and the Caspian Sea: A microcosm experiment
Changes in the microbial community in response to catastrophic oil spills in marine and fresh water environments have been well documented. Molecular methods provide tools for analyzing the entire bacterial community, covering also those bacteria that have not been cultured in the laboratory. In this study, four different microcosms were set up containing sediments collected from the Persian Gu...
متن کاملThe effects of crude oil on marine microbial communities in sediments from the Persian Gulf and the Caspian Sea: A microcosm experiment
Changes in the microbial community in response to catastrophic oil spills in marine and fresh water environments have been well documented. Molecular methods provide tools for analyzing the entire bacterial community, covering also those bacteria that have not been cultured in the laboratory. In this study, four different microcosms were set up containing sediments collected from the Persian Gu...
متن کاملAn improved fluorescence in situ hybridization protocol for the identification of bacteria and archaea in marine sediments.
In situ identification of prokaryotic cells in subsurface sediments is hampered by the low cellular rRNA contents of the target organisms. Fluorescence in situ hybridization with catalyzed reporter deposition (CARD-FISH) has the potential to overcome this limitation, and was therefore optimized for a 40 cm deep sediment core sampled from a tidal sandy flat of the German Wadden Sea. Treatment wi...
متن کاملFluorescence in situ hybridization and catalyzed reporter deposition for the identification of marine bacteria.
Fluorescence in situ hybridization (FISH) with horseradish peroxidase (HRP)-labeled oligonucleotide probes and tyramide signal amplification, also known as catalyzed reporter deposition (CARD), is currently not generally applicable to heterotrophic bacteria in marine samples. Penetration of the HRP molecule into bacterial cells requires permeabilization procedures that cause high and most proba...
متن کاملQuantification of Microbial Communities in Subsurface Marine Sediments of the Black Sea and off Namibia
Organic-rich subsurface marine sediments were taken by gravity coring up to a depth of 10 m below seafloor at six stations from the anoxic Black Sea and the Benguela upwelling system off Namibia during the research cruises Meteor 72-5 and 76-1, respectively. The quantitative microbial community composition at various sediment depths was analyzed using total cell counting, catalyzed reporter dep...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 64 7 شماره
صفحات -
تاریخ انتشار 1998